Nathan Fillion

Malcolm 'Mal' Reynolds

Summer Glau

River Tam

Gina Torres

Zoë Alleyne Washburne

Alan Tudyk

Hoban 'Wash' Washburne

Morena Baccarin

Inara Serra

Adam Baldwin

Jayne Cobb

Jewel Staite

Kaywinnet Lee 'Kaylee' Frye

Sean Maher

Simon Tam

Ron Glass

Shepherd Derrial Book

Chiwetel Ejiofor

The Operative

David Krumholtz

Mr. Universe

Michael Hitchcock

Dr. Mathias

Sarah Paulson

Dr. Caron

Yan Feldman

Mingojerry 'Mingo' Rample

Rafael Feldman

Fantastic "Fanty" Rample

Nectar Rose

Lenore

Tamara Taylor

Teacher

Glenn Howerton

Lilac Young Tough

Hunter Ansley Wryn

Young River Tam

Logan O'Brien

Boy Student

Erik Erotas

Boy Student

Demetra Raven

Girl Student

Marley McClean

Girl Student

Jessica Huang

Girl Student

Scott Kinworthy

Ensign

Erik Weiner

Helmsman

Conor O'Brien

Lab Technician

Peter James Smith

Lab Technician

Weston Nathanson

Trade Agent

Carrie "CeCe" Cline

Young Female Intern

Chuck O'Neil

Vault Guard

Amy Wieczorek

Lilac Mom

Tristan Jarred

Lilac Son

Elaine Mani Lee

Fan Dancer

Terrence Hardy Jr.

Mining Camp Boy

Brian O'Hare

Alliance Pilot

Ryan Tasz

Black Room Soldier

Colin Patrick Lynch

Black Room Soldier

Terrell Tilford

News Anchor

Joshua Michael Kwiat

Slovenly Beaumonde Man

Antonio Rufino

Bartender (uncredited)

Linda Wang

Chinese Snake Dancer (uncredited)

Rick Williamson

Bar Guy (uncredited)

Neil Patrick Harris

Screaming Reaver (uncredited)

Matt McColm

Lab Security (uncredited)

Marcus Young

Bar Security (uncredited)

Dennis Keiffer

Bar Guy (uncredited)