
Gustafsson was born in Goteborg, Sweden. He studied at the Calle Flygares Theater School in Stockholm to become an actor. But before his debut as an actor, he had already debuted in 2005 as a stand-up comedian at Bungy Comedy, a Swedish club for stand-up beginners. In 2006 he enrolled at Standup Star, a stand-up school, where among others, Özz Nûjen and Jakob Öqvist, two of the most well-known comedians in Sweden, have attended.
As of 2008, his most notable appearance was in Melodifestivalen 2008, the Swedish selections for the Eurovision Song Contest. For example, he sang a song to Kristian Luuk's (the host) girlfriend Carina Berg. In his song he declared his love for her. Afterward, Berg said that she had to hide her cheeks so people couldn't see that she was blushing.
Gustafsson gained a certain degree of international fame following a World of Warcraft (WoW) joke in Parlamentet, talking about people wasting time on the internet and not contributing to their party's effort by playing inefficiently, using so much internal WoW terminology himself that he was incomprehensible to non-players. The scene was spread over the Internet, mainly on YouTube, where translations into other languages could also be found.
In 2008, Björn received big media attention by great efforts in Parlamentet and as an entertainment in the breaks to Melodifestivalens and he also got voted as the Sweden's funniest man by Aftonbladet's readers on 6 March 2008. He also read the Swedish votes at the Eurovision Song Contest 2008. He continued his "stand-up" shows at the big stage, in the 44th Guldbagge Awards in 2009 together with Johan Glans, and with Robert Gustafsson in the Svenska idrottsgalan 2009 (Swedish athletics awards) where he also presented the nominations for rookie of the year.

The Penguin Lessons
Tapio
2025

Björnzone 2.0
Self
2024

Koppla av
Various · (10 episodes)
2024

Ture Sventon och den magiska lampan
Polisen Pär · (6 episodes)
2023

Padel Dreams
Fabian · (6 episodes)
2023

Clark
Kaj-Robert · (6 episodes)
2022

Till Sun Rises
Henrik
2021

Alla utom vi
Ola · (20 episodes)
2021

Carina Bergfeldt
Self - Guest · (1 episode)
2021

Ture Sventon och jakten på ungdomens källa
Agent-Pär · (10 episodes)
2021

Nelly Rapp – Monster Agent
Vincent
2020

Bert's Diary
Lynx / Lo
2020

Ture Sventon and the Secrets of The Bermuda Triangle
Per · (6 episodes)
2019

Call Mom!
Manlig terapeut
2019

Kingdom of Sweden
Various
2018

Becoming Astrid
Sture
2018

Becoming Astrid
Sture Lindgren
2018

Skratta Då: En Rolig Historia Om Svensk Standup
Self
2017

People of Earth
Don the White · (20 episodes)
2016

Kung Fury
Private Lahmstache
2015

Spy
Anton
2015

Other Space
(1 episode)
2015

Barna Hedenhös uppfinner julen
Junior · (24 episodes)
2013

Svensk humor
Various roles · (10 episodes)
2013

Historieätarna
Himself · (1 episode)
2012

Historieätarna
Self · (7 episodes)
2012

Tina & Bettina: The Movie
Niklas
2012

Cockpit
Albin
2012

Kontoret
Viking · (18 episodes)
2012

Nissene over skog og hei
Håkan Holmgren · (24 episodes)
2011

The Crown Jewels
Pettersson-Jonsson
2011

Klown
Male Host - Castello Alleycat
2010
Roast på Berns
(1 episode)
2009

Kenny Begins
Lenny Starfighter
2009

Grown Ups
Johan
2008

Morgonsoffan
(12 episodes)
2008

Beck 18 - The Girl in the Root Cellar
2006

Robins
Self - Guest · (1 episode)
2006

Melodifestivalen
Self · (12 episodes)
1959